A structural remedy toward bright dipolar fluorophores in aqueous media† †Electronic supplementary information (ESI) available: Materials and procedures regarding the synthesis of all the dyes and the biothiol probes, one-photon and two-photon spectroscopic analysis, tissue and cell imaging experiments, and theoretical computations. See DOI: 10.1039/c5sc01076d

Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes† †Electronic supplementary information (ESI) available: Materials and procedures regarding the synthesis of all of the dyes, photophysical properties and tissue and cell imaging. See DOI: 10.1039/c7sc03362a

The fluorescence imaging of tissue is essential for studying biological events beyond the cellular level. Twophoton microscopy based on the nonlinear light absorption of fluorescent dyes is a viable tool for the high resolution imaging of tissue. A key limitation for deep tissue imaging is the autofluorescence from intrinsic biomolecules. Here, we report a systematic study that discloses relati...

Green- to far-red-emitting fluorogenic tetrazine probes – synthetic access and no-wash protein imaging inside living cells† †Electronic supplementary information (ESI) available: Synthetic procedures and spectroscopic data, details of cell experiments and imaging. See DOI: 10.1039/c6sc03879d Click here for additional data file.

Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce background signal in fluorescence microscopy imaging. 1,2,4,5-Tetrazines are known substrates for the bioorthogonal inverse electron demand Diels–Alder reaction (DAinv) and tetrazine substituted fluorophores can exhibit fluorogenic properties. Herein, we report the synthesis of a palette of novel fluorogenic...

Engineering a FRET strategy to achieve a ratiometric two-photon fluorescence response with a large emission shift and its application to fluorescence imaging† †Electronic supplementary information (ESI) available: Synthetic procedures and characterization data; additional spectroscopic data. See DOI: 10.1039/c4sc03883e Click here for additional data file.

Aminomaleimide fluorophores: a simple functional group with bright, solvent dependent emission† †Electronic supplementary information (ESI) available: Experimental protocol, supplementary figures and additional spectroscopic data. See DOI: 10.1039/c5cc02908b Click here for additional data file.

A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue† †Electronic supplementary information (ESI) available: General methods, synthetic procedures, NMR spectra, kinetic data, pH profile, metal selectivity graph, cell culture and staining protocols, wide-field microscopy experiments, tissue specimen preparation, and two-photon imaging techniques. See DOI: 10.1039/c4sc03388d Click here for additional data file.

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn2+ in lysosomes of HeLa cells, endogenous Zn2+ in insulin granules of MIN6 cells...